Evaluation of Real Time Cell Proliferation, Anti-Inflammatory and Wound Healing Potential of Helenalin on HaCaT Keratinocytes Treated with Lipopolysaccharide Stimulated Monocytes

Arnica montana, of the family Asteraceae, is used for its anti-inflammatory and wound healing properties, especially bruises, tissue injuries other traumas. However, cellular molecular mechanisms are not yet fully known. Its main active ingredient helenalin, a sesquiterpene lactone, known as one ingredients Asteraceae species. The aim this study was to investigate cell proliferation, activity helenalin on human keratinocyte cells treated with lipopolysaccharide stimulated monocytic line. Human were different concentrations (0.095-50 μM) determine toxic nontoxic concentrations. Then, effects (0.02 0.2 (2 proliferation migration determined using real time analysis system. scratch assay performed supernatants collected cytokine levels under inflammatory non-inflammatory conditions. Under conditions, 0.02 μM increased induced most in cells. Cell migration, higher exposed than μM. Helenalin has been found decrease production cytokines, conditions increase closure by increasing migration.